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bovine thrombin  (Pfizer Inc)

 
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    Pfizer Inc bovine thrombin
    Bovine Thrombin, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bovine+thrombin/pmc13148753-13-7-4?v=Pfizer+Inc
    Average 86 stars, based on 1 article reviews
    bovine thrombin - by Bioz Stars, 2026-07
    86/100 stars

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    Flow cytometry analysis of platelet activation by surface P‐selectin expression (CD62P) in six canine platelet concentrate units stored at 4°C for 14 days. (A) In unstimulated (resting) platelets, platelet activation was measured as percentage of P‐selectin‐positive cells and P‐selectin surface density, measured as median fluorescence intensity (MFI). Over time, an increased number of P‐selectin‐positive platelets was detected and was significantly higher on Days 12 and 14. However, this did not translate to increased MFI over time since P‐selectin MFI was lower from Day 9. Platelet response to the agonists (B) adenosine diphosphate (ADP), (C) convulxin (CVX), and <t>(D)</t> <t>thrombin</t> (Throm) was measured by fold change (FC) in MFI (log10) relative to resting platelets. (B) Response to ADP was minimal until Days 12 and 14. (C) Compared to ADP, convulxin was able to elicit platelet activation throughout the storage period with maximum response noted on Day 7. (D) A decline in response to thrombin was noted. After Day 7, P‐selectin upregulation in response to thrombin was minimal and significantly less compared to Day 0.
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    Flow cytometry analysis of platelet activation by surface P‐selectin expression (CD62P) in six canine platelet concentrate units stored at 4°C for 14 days. (A) In unstimulated (resting) platelets, platelet activation was measured as percentage of P‐selectin‐positive cells and P‐selectin surface density, measured as median fluorescence intensity (MFI). Over time, an increased number of P‐selectin‐positive platelets was detected and was significantly higher on Days 12 and 14. However, this did not translate to increased MFI over time since P‐selectin MFI was lower from Day 9. Platelet response to the agonists (B) adenosine diphosphate (ADP), (C) convulxin (CVX), and (D) thrombin (Throm) was measured by fold change (FC) in MFI (log10) relative to resting platelets. (B) Response to ADP was minimal until Days 12 and 14. (C) Compared to ADP, convulxin was able to elicit platelet activation throughout the storage period with maximum response noted on Day 7. (D) A decline in response to thrombin was noted. After Day 7, P‐selectin upregulation in response to thrombin was minimal and significantly less compared to Day 0.

    Journal: Journal of Veterinary Emergency and Critical Care

    Article Title: Assessment of Platelet Storage Lesions, Viability, and Function in Canine Platelet Concentrate Units Stored at 4°C for 14 Days

    doi: 10.1111/vec.13470

    Figure Lengend Snippet: Flow cytometry analysis of platelet activation by surface P‐selectin expression (CD62P) in six canine platelet concentrate units stored at 4°C for 14 days. (A) In unstimulated (resting) platelets, platelet activation was measured as percentage of P‐selectin‐positive cells and P‐selectin surface density, measured as median fluorescence intensity (MFI). Over time, an increased number of P‐selectin‐positive platelets was detected and was significantly higher on Days 12 and 14. However, this did not translate to increased MFI over time since P‐selectin MFI was lower from Day 9. Platelet response to the agonists (B) adenosine diphosphate (ADP), (C) convulxin (CVX), and (D) thrombin (Throm) was measured by fold change (FC) in MFI (log10) relative to resting platelets. (B) Response to ADP was minimal until Days 12 and 14. (C) Compared to ADP, convulxin was able to elicit platelet activation throughout the storage period with maximum response noted on Day 7. (D) A decline in response to thrombin was noted. After Day 7, P‐selectin upregulation in response to thrombin was minimal and significantly less compared to Day 0.

    Article Snippet: Bovine α‐thrombin, Prolytix, Essex Junction, VT.

    Techniques: Flow Cytometry, Activation Assay, Expressing, Fluorescence

    Flow cytometry analysis of phosphatidylserine (PS) externalization, detected on the platelet surface, in six canine platelet concentrate units stored at 4°C for 14 days. (A) In unstimulated platelets, the percentage of Annexin V‐positive platelets decreased on Days 5, 7, and 9 compared to Day 0. (B) To detect the ability of platelets to externalize PS, platelets were activated with thrombin (Thromb) in the presence of calcium. Percentage change in Annexin V compared to resting platelets (AV % change) remained static and close to zero throughout storage. (C) Compared to the positive controls of A23187‐treated platelets, PS externalization occurred throughout the storage period and increased on Days 7 and 9 from Day 0.

    Journal: Journal of Veterinary Emergency and Critical Care

    Article Title: Assessment of Platelet Storage Lesions, Viability, and Function in Canine Platelet Concentrate Units Stored at 4°C for 14 Days

    doi: 10.1111/vec.13470

    Figure Lengend Snippet: Flow cytometry analysis of phosphatidylserine (PS) externalization, detected on the platelet surface, in six canine platelet concentrate units stored at 4°C for 14 days. (A) In unstimulated platelets, the percentage of Annexin V‐positive platelets decreased on Days 5, 7, and 9 compared to Day 0. (B) To detect the ability of platelets to externalize PS, platelets were activated with thrombin (Thromb) in the presence of calcium. Percentage change in Annexin V compared to resting platelets (AV % change) remained static and close to zero throughout storage. (C) Compared to the positive controls of A23187‐treated platelets, PS externalization occurred throughout the storage period and increased on Days 7 and 9 from Day 0.

    Article Snippet: Bovine α‐thrombin, Prolytix, Essex Junction, VT.

    Techniques: Flow Cytometry

    Light transmission aggregometry, evaluating platelet aggregation in response to thrombin, ADP, or collagen (COL) in canine platelet concentrate stored at 4°C for 14 days. Minimal aggregation response to ADP and COL in maximum aggregation (%) and slope was noted throughout the storage period, while response to thrombin was observed until Day 14.

    Journal: Journal of Veterinary Emergency and Critical Care

    Article Title: Assessment of Platelet Storage Lesions, Viability, and Function in Canine Platelet Concentrate Units Stored at 4°C for 14 Days

    doi: 10.1111/vec.13470

    Figure Lengend Snippet: Light transmission aggregometry, evaluating platelet aggregation in response to thrombin, ADP, or collagen (COL) in canine platelet concentrate stored at 4°C for 14 days. Minimal aggregation response to ADP and COL in maximum aggregation (%) and slope was noted throughout the storage period, while response to thrombin was observed until Day 14.

    Article Snippet: Bovine α‐thrombin, Prolytix, Essex Junction, VT.

    Techniques: Transmission Assay